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Established in 1996, the Molecular Genomics Core (MGC) is a state-of-the-art facility offering a variety of experimental platforms to facilitate genomics research. Accredited as a Duke Shared Resource facility in 2007, the MGC offers decades of experience with genetic, genomic and epigenomic study design and technology, working closely with researchers to customize experiments to meet their needs. The MGC highlights applications that include 10x Genomics NGS library generation for both single cell and gDNA experiments, DNA methylation microarrays, SNP genotyping and copy number microarrays, and Taqman targeted SNP genotyping.

 

 

PUBLICATION HIGHLIGHTS

This paper is highlighted on the cover of Molecular Cancer Research.
CRISPR Editing of Mutant IDH1 R132H Induces a CpG Methylation-Low State in Patient-Derived Glioma Models of G-CIMP
Moure CJ, Diplas BH, Chen LH, Yang R, Pirozzi CJ, Wang Z, Spasojevic I, Waitkus MS, He Y, Yan H.
Mol Cancer Res. 2019 Oct;17(10):2042-2050. doi: 10.1158/1541-7786.MCR-19-0309. Epub 2019 Jul 10.

Single cell RNA sequencing identifies YAP1-dependent hepatic mesothelial progenitors in fibrolamellar carcinoma.
Jewell ML, Gibson JR, Guy CD, Hyun J, Du K, Oh SH, Premont RT, Hsu DS, Ribar T, Gregory SG, Diehl AM.
Am J Pathol. 2019 Oct 24. pii: S0002-9440(19)30770-9. doi: 10.1016/j.ajpath.2019.09.018. [Epub ahead of print] PMID: 31669305

Microglial Function Is Distinct in Different Anatomical Locations during Retinal Homeostasis and Degeneration
Emily G. O’Koren, Chen Yu, Mikael Klingeborn, Alicia Y.W. Wong,  Cameron L. Prigge, Rose Mathew, Joan Kalnitsky, Rasha A. Msallam, Aymeric Silvin, Jeremy N. Kay, Catherine Bowes Rickman, Vadim Y. Arshavsky, Florent Ginhoux, Miriam Merad, Daniel R. Saban
Immunity. Volume 50, Issue 3, 19 March 2019, Pages 723-737.e7

Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function
Rebekah E. Dumm, Jessica K. Fiege, Barbara M. Waring, Chay T. Kuo, Ryan A. Langlois & Nicholas S. Heaton
Nature Communications. Volume 10, Article number: 779 (2019).

Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular osteopontin
Kanayama M, Xu S, Danzaki K, Gibson JR, Inoue M, Gregory SG2 Shinohara ML.
Nat Immunol. 2017 Sep;18(9):973-984.

A Missense Variant in PTPN22 is a Risk Factor for Drug-induced Liver Injury.
Cirulli ET, Nicoletti P, Abramson K, Andrade RJ, Bjornsson ES, Chalasani N, Fontana RJ, Hallberg P, Li YJ, Lucena MI, Long N, Molokhia M, Nelson MR, Odin JA, Pirmohamed M, Rafnar T, Serrano J, Stefansson K, Stolz A, Daly AK, Aithal GP, Watkins PB; Drug-Induced Liver Injury Network (DILIN) investigators and International DILI consortium (iDILIC)
Gastroenterology. 2019 May;156(6):1707-1716.e2. doi: 10.1053/j.gastro.2019.01.034. Epub 2019 Jan 18.

 

ANNOUNCEMENTS

  • Be sure to download your raw scRNAseq data from the GCB for publishing. The MGC does not guarantee retention of raw sequencing data after 3 months from delivering data analysis.