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Spatial Gene Expression
The relationship between cells and their organization within tissue is critical to understanding normal development and disease pathology. The Visium Spatial Gene Expression Solution incorporates unbiased, high-throughput total mRNA analysis for intact tissue sections with morphological context, offering a previously inaccessible view of tissue biology. Preserving spatial context while identifying distinct groups of cells offers critical information to understand the relationship of cellular function, phenotype, and location in tissue microenvironments.
Fresh-frozen tissue samples are sectioned and placed in the four Capture Areas on the Visium Spatial Gene Expression slide. Utilizing standard fixation and staining techniques, including Hematoxylin and Eosin (H&E) staining, tissue sections are visualized on slides. The tissue is then permeabilized to release mRNA from the cells. mRNA binds with spatially-barcoded oligonucleotides present on the spots. A reverse transcription reaction produces cDNA from captured mRNA. The second strand of cDNA is then synthesized and denatured. The barcoded cDNA is then pooled for downstream processing and library preparation to complete a sequencing-ready library. The Visium Spatial Gene Expression library is sequenced using standard short-read sequencers, and data is processed and visualized using 10x Genomics software: Space Ranger Analysis Pipelines and Loupe Browser.